Hotspot

rpl16

LSC · 80201-81676 · 1476 bp

Back to Pilea

Pi 0.0444
MarkerSeek score 81.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1476
variable_sites
233
pi
0.0444
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TTTCTGGCTCCGCTGTTATC GAGTTTCGTTTCGCAAAGCA 50.0 45.0 58.0 57.9 2008-2050 bp 0.6875 78.5
rpl16_p2 TTTCTGGCTCCGCTGTTATC ACCGGAAACTAAACGTTGCT 50.0 45.0 58.0 58.0 2102-2141 bp 0.1875 49.2
rpl16_p3 TTTCTGGCTCCGCTGTTATC TGCCTCTACAAACCATTCGG 50.0 50.0 58.0 57.9 1871-1910 bp 0.1875 48.9
rpl16_p4 ACGTGAGACACAAACAAGCT ACCGGAAACTAAACGTTGCT 45.0 45.0 58.0 58.0 2509-2548 bp 0.1875 48.8
rpl16_p5 ACGTGAGACACAAACAAGCT GAGTTTCGTTTCGCAAAGCA 45.0 45.0 58.0 57.9 2416-2455 bp 0.1875 48.5