Hotspot

trnT-UGU-trnL-UAA

LSC · 46358-46927 · 570 bp

Back to Pilea

Pi 0.0839
MarkerSeek score 86.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
570
variable_sites
161
pi
0.0839
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CTAACCTCTGAGCTAAGCGG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.5 58.2 626-770 bp 1.0 86.1
trnT-UGU-trnL-UAA_p2 CTAACCTCTGAGCTAAGCGG TAAGTCCGTAGCGTCTACCA 55.0 50.0 57.5 57.6 628-772 bp 1.0 84.8
trnT-UGU-trnL-UAA_p3 CTAACCTCTGAGCTAAGCGG AAGTCCGTAGCGTCTACCAA 55.0 50.0 57.5 58.5 627-771 bp 1.0 84.3
trnT-UGU-trnL-UAA_p4 CTAACCTCTGAGCTAAGCGG CGTCTACCAATTCCGCCATA 55.0 50.0 57.5 57.5 617-761 bp 1.0 83.8
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.5 58.2 623 bp 0.0625 37.3