Hotspot

atpH-atpI

LSC · 13419-14091 · 673 bp

Pi 0.0605

MarkerSeek score 83.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 144
pi: 0.0605
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCAGAAATCAGCGGATTCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.6	58.5	723-782 bp	1.0	84.6
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	732-791 bp	0.9375	84.5
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	744-803 bp	0.9375	83.3
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.6	58.5	746-805 bp	0.9375	82.0
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.6	58.5	745-804 bp	0.9375	82.0