Hotspot

rps16-trnQ-UUG

LSC · 6055-6752 · 698 bp

Back to Pilea

Pi 0.112
MarkerSeek score 82.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
698
variable_sites
252
pi
0.112
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 423-877 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 444-898 bp 1.0 86.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 427-881 bp 1.0 85.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 453-907 bp 1.0 85.4
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 415-869 bp 1.0 85.1