Hotspot

trnK-UUU-rps16

LSC · 4177-4876 · 700 bp

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Pi 0.0748
MarkerSeek score 83.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
700
variable_sites
171
pi
0.0748
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGACATGCTGCTTTTTCCAG ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.0 58.0 821-998 bp 0.75 81.7
trnK-UUU-rps16_p2 CGACATGCTGCTTTTTCCAG AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1490-1690 bp 0.75 81.7
trnK-UUU-rps16_p3 AGTCGCGGTCTTACAAACTC ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 783-961 bp 0.75 81.5
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 782-960 bp 0.75 81.5
trnK-UUU-rps16_p5 CGACATGCTGCTTTTTCCAG AAAGGGTATGTTGCTGCCAA 50.0 45.0 58.0 58.0 1590-1613 bp 0.1875 48.8