primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	GGCATTTCCACAATGACTGC	CTTTGATTTGATTGCGCCGT	20	20	50.0	45.0	58.0	58.0	0.0	4.2	0.019176	3378	4139	1203-1210	0.187500	48.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	TGAGAAGATTCGTTGCGGAG	CTTTGATTTGATTGCGCCGT	20	20	50.0	45.0	58.0	58.0	0.0	4.2	0.022455	3378	4139	1581-1588	0.187500	48.1
matK-trnK-UUU_p3	matK-trnK-UUU	3	GTCGTAGGGAACATGGCATT	CTTTGATTTGATTGCGCCGT	20	20	50.0	45.0	58.0	58.0	0.0	4.2	0.057133	3378	4139	1217-1224	0.187500	47.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	GGCATTTCCACAATGACTGC	CGCTTCGATATGTAGTGCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.134631	3378	4139	1540-1556	0.187500	47.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCTCTGCCCGTTAAGGAAGA	CTTTGATTTGATTGCGCCGT	20	20	50.0	45.0	58.1	58.0	0.0	4.2	0.085725	3378	4139	1242-1249	0.187500	47.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGACATGCTGCTTTTTCCAG	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.031485	4177	4876	821-998	0.750000	81.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGACATGCTGCTTTTTCCAG	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.035104	4177	4876	1490-1690	0.750000	81.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.066364	4177	4876	783-961	0.750000	81.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.066364	4177	4876	782-960	0.750000	81.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGACATGCTGCTTTTTCCAG	AAAGGGTATGTTGCTGCCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.035104	4177	4876	1590-1613	0.187500	48.8
rps16_p1	rps16	1	ACGGCGCAATCAAATCAAAG	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	10.8	16.9	0.094847	4877	6054	2198-2536	0.187500	52.7
rps16_p2	rps16	2	TGGCACTACATATCGAAGCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.210302	4877	6054	1853-2199	0.187500	51.8
rps16_p3	rps16	3	ACGGCGCAATCAAATCAAAG	GCGACTTCGTCAGAAACTCT	20	20	45.0	50.0	58.0	57.9	10.8	0.0	0.072028	4877	6054	1948-1971	0.187500	48.7
rps16_p4	rps16	4	TGGCACTACATATCGAAGCG	GCGACTTCGTCAGAAACTCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.187483	4877	6054	1611-1625	0.187500	47.6
rps16_p5	rps16	5	ACGGCGCAATCAAATCAAAG	TTCCGTCCCAGAGCATATCT	20	20	45.0	50.0	58.0	57.9	10.8	0.0	0.106851	4877	6054	2523-2523	0.062500	41.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6055	6752	423-877	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.672468	6055	6752	444-898	1.000000	86.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6055	6752	427-881	1.000000	85.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6055	6752	453-907	1.000000	85.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6055	6752	415-869	1.000000	85.1
atpH-atpI_p1	atpH-atpI	1	AGCAGAAATCAGCGGATTCA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.6	58.5	12.6	0.0	0.925993	13419	14091	723-782	1.000000	84.6
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.621503	13419	14091	732-791	0.937500	84.5
atpH-atpI_p3	atpH-atpI	3	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.3	58.5	0.0	0.0	0.779884	13419	14091	744-803	0.937500	83.3
atpH-atpI_p4	atpH-atpI	4	AATCCAGCAGCAATAACGGA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.6	58.5	0.0	0.0	0.936090	13419	14091	746-805	0.937500	82.0
atpH-atpI_p5	atpH-atpI	5	ATCCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.6	58.5	0.0	0.0	0.936090	13419	14091	745-804	0.937500	82.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ACCTACCCCCTTTTAGTCCT	TAGTATGATGGCGGTTGTGC	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.111480	29428	29922	1801-1814	0.187500	40.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ACCTACCCCCTTTTAGTCCT	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.156695	29428	29922	1781-1794	0.187500	39.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACCTACCCCCTTTTAGTCCT	TAGCGGTTCAGGACATCTCT	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.204982	29428	29922	1763-1776	0.187500	39.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ACCTACCCCCTTTTAGTCCT	TCTAGCGGTTCAGGACATCT	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.204982	29428	29922	1765-1778	0.187500	39.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ACCTACCCCCTTTTAGTCCT	GTCTAGCGGTTCAGGACATC	20	20	50.0	55.0	56.9	57.8	0.0	0.0	1.270937	29428	29922	1766-1779	0.187500	38.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30611	31191	575-681	1.000000	90.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30611	31191	573-679	1.000000	90.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30611	31191	554-660	1.000000	90.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30611	31191	552-658	1.000000	90.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	30611	31191	572-678	1.000000	90.3
ycf3-trnS-GCU_p1	ycf3-trnS-GCU	1	ACTTCACCTTCAACAAGCGT	AAGAAAGGTGCCCGTGATTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.043011	44181	44802	1062-1069	0.187500	49.1
ycf3-trnS-GCU_p2	ycf3-trnS-GCU	2	CCAACCCTCTTGGAAAAGCT	AAGAAAGGTGCCCGTGATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.005700	44181	44802	1490-1498	0.187500	49.0
ycf3-trnS-GCU_p3	ycf3-trnS-GCU	3	CCAACCCTCTTGGAAAAGCT	GGTGCCCGTGATTTGATTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006160	44181	44802	1484-1492	0.187500	49.0
ycf3-trnS-GCU_p4	ycf3-trnS-GCU	4	TATTTGTCTTAAGCGCCGCT	AAGAAAGGTGCCCGTGATTT	20	20	45.0	45.0	58.0	58.0	5.4	0.0	0.023166	44181	44802	1413-1419	0.187500	48.8
ycf3-trnS-GCU_p5	ycf3-trnS-GCU	5	TATTTGTCTTAAGCGCCGCT	GGTGCCCGTGATTTGATTTG	20	20	45.0	50.0	58.0	58.0	5.4	0.0	0.023626	44181	44802	1407-1413	0.187500	48.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CTAACCTCTGAGCTAAGCGG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.5	58.2	5.5	10.2	0.740317	46358	46927	626-770	1.000000	86.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CTAACCTCTGAGCTAAGCGG	TAAGTCCGTAGCGTCTACCA	20	20	55.0	50.0	57.5	57.6	5.5	10.2	0.902092	46358	46927	628-772	1.000000	84.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CTAACCTCTGAGCTAAGCGG	AAGTCCGTAGCGTCTACCAA	20	20	55.0	50.0	57.5	58.5	5.5	10.2	0.962461	46358	46927	627-771	1.000000	84.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CTAACCTCTGAGCTAAGCGG	CGTCTACCAATTCCGCCATA	20	20	55.0	50.0	57.5	57.5	5.5	0.0	1.019885	46358	46927	617-761	1.000000	83.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCAATTCC	20	20	55.0	55.0	57.5	58.2	5.5	10.2	0.651412	46358	46927	623-623	0.062500	37.3
rbcL-accD_p1	rbcL-accD	1	GGCTTCAGGGGGTATTCATG	ATAGGATCCCAAGTACCCGG	20	20	55.0	55.0	58.0	58.0	0.0	8.6	0.038753	55898	56650	1791-1833	0.937500	87.0
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	ATAGGATCCCAAGTACCCGG	20	20	50.0	55.0	58.0	58.0	0.0	8.6	0.055474	55898	56650	1628-1670	0.937500	87.0
rbcL-accD_p3	rbcL-accD	3	GCCGTAGCTAATCGAGTAGC	ATAGGATCCCAAGTACCCGG	20	20	55.0	55.0	58.0	58.0	31.7	8.6	0.059214	55898	56650	1673-1712	0.750000	79.3
rbcL-accD_p4	rbcL-accD	4	GGCTTCAGGGGGTATTCATG	TGGGCCACACCTGTATTCTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.053408	55898	56650	1108-1114	0.187500	48.8
rbcL-accD_p5	rbcL-accD	5	TCGGTGGAGGAACTTTAGGT	ATAGGATCCCAAGTACCCGG	20	20	50.0	55.0	58.0	58.0	0.0	8.6	0.045443	55898	56650	1720-1726	0.187500	48.4
rpl16_p1	rpl16	1	TTTCTGGCTCCGCTGTTATC	GAGTTTCGTTTCGCAAAGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.123809	80201	81676	2008-2050	0.687500	78.5
rpl16_p2	rpl16	2	TTTCTGGCTCCGCTGTTATC	ACCGGAAACTAAACGTTGCT	20	20	50.0	45.0	58.0	58.0	0.0	9.5	0.066507	80201	81676	2102-2141	0.187500	49.2
rpl16_p3	rpl16	3	TTTCTGGCTCCGCTGTTATC	TGCCTCTACAAACCATTCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132126	80201	81676	1871-1910	0.187500	48.9
rpl16_p4	rpl16	4	ACGTGAGACACAAACAAGCT	ACCGGAAACTAAACGTTGCT	20	20	45.0	45.0	58.0	58.0	0.0	9.5	0.066507	80201	81676	2509-2548	0.187500	48.8
rpl16_p5	rpl16	5	ACGTGAGACACAAACAAGCT	GAGTTTCGTTTCGCAAAGCA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.123809	80201	81676	2416-2455	0.187500	48.5
ndhF-rpl32_p1	ndhF-rpl32	1	CCGGGTGAACCAAAAACCTA	AGACTGACTGCTTTTCGAATCA	20	22	50.0	40.9	58.0	58.0	1.2	0.0	2.020735	110750	111781	1282-1845	0.750000	65.8
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCGTGGGAAAAAGTA	AGACTGACTGCTTTTCGAATCA	20	22	45.0	40.9	57.9	58.0	0.0	0.0	2.059033	110750	111781	1330-1440	0.625000	60.5
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCCGTGGGAAAAAGTA	AGTGGCTGCAAATTTCCTATCT	20	22	45.0	40.9	57.9	58.0	0.0	0.5	2.080807	110750	111781	1823-1837	0.187500	33.2
ndhF-rpl32_p4	ndhF-rpl32	4	CCGGGTGAACCAAAAACCTA	AGTGGCTGCAAATTTCCTATCT	20	22	50.0	40.9	58.0	58.0	1.2	0.5	2.042509	110750	111781	2228-2242	0.187500	33.1
ndhF-rpl32_p5	ndhF-rpl32	5	CCGGGTGAACCAAAAACCTA	GTGGCTGCAAATTTCCTATCTG	20	22	50.0	45.5	58.0	58.0	1.2	0.5	2.053792	110750	111781	2227-2241	0.187500	33.0
ycf1_p1	ycf1	1	TACATTTTACGACCTGGCGG	GCCGTTCGGTATGATGGTTA	20	20	50.0	50.0	58.0	57.8	13.5	12.8	0.241074	123112	123231	533-533	0.187500	46.2
ycf1_p2	ycf1	2	TACATTTTACGACCTGGCGG	TATGCCGTTCGGTATGATGG	20	20	50.0	50.0	58.0	57.6	13.5	21.2	0.461863	123112	123231	536-536	0.187500	44.4
ycf1_p3	ycf1	3	GGCCTTTTTCTTGGTCCCTT	GCCGTTCGGTATGATGGTTA	20	20	50.0	50.0	58.3	57.8	0.0	12.8	0.511106	123112	123231	920-920	0.187500	44.1
ycf1_p4	ycf1	4	ATGGCCTTTTTCTTGGTCCC	GCCGTTCGGTATGATGGTTA	20	20	50.0	50.0	58.4	57.8	3.3	12.8	0.583628	123112	123231	922-922	0.187500	43.6
ycf1_p5	ycf1	5	TGGCGGTTCGTGTATGTTTT	GCCGTTCGGTATGATGGTTA	20	20	45.0	50.0	58.4	57.8	0.0	12.8	0.632671	123112	123231	519-519	0.187500	43.1