Hotspot

ycf1

Genome · 98305-103638 · 5334 bp

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Pi 0.00586
MarkerSeek score 49.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
5334
variable_sites
107
pi
0.00586
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GCAGCTGTGATGGAACCTTA ACATTGTCTCTTCGCGTCTC 50.0 50.0 57.9 58.0 482-698 bp 1.0 86.4
ycf1_p2 GCAGCTGTGATGGAACCTTA CATTGTCTCTTCGCGTCTCA 50.0 50.0 57.9 58.0 481-697 bp 1.0 86.4
ycf1_p3 ACCGGTACAACATATCCCCA ACATTGTCTCTTCGCGTCTC 50.0 50.0 58.1 58.0 613-829 bp 1.0 85.8
ycf1_p4 ACCGGTACAACATATCCCCA CATTGTCTCTTCGCGTCTCA 50.0 50.0 58.1 58.0 612-828 bp 1.0 85.8
ycf1_p5 GCAGCTGTGATGGAACCTTA TTACCGGCTTTATCGTTCCG 50.0 50.0 57.9 58.1 752-968 bp 1.0 85.5