Hotspot

rps7-trnL-CAA

Genome · 114565-116151 · 1587 bp

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Pi 0.00677
MarkerSeek score 60.30
Primer available yes
Label rps7-trnL-CAA

Feature Metadata

label_name
rps7-trnL-CAA
feature_type
igs
region
Genome
length_bp
1587
variable_sites
39
pi
0.00677
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-trnL-CAA_p1 CAGAGGGAATGGCAATGCTA GTCTCGCGTGTCTACCATTT 50.0 50.0 57.6 58.0 1669-1704 bp 1.0 81.7
rps7-trnL-CAA_p2 CAGAGGGAATGGCAATGCTA CTCGCGTGTCTACCATTTCA 50.0 50.0 57.6 58.0 1667-1702 bp 1.0 81.7
rps7-trnL-CAA_p3 TTAATGGATGCTGCCAGAGG GTCTCGCGTGTCTACCATTT 50.0 50.0 57.6 58.0 1683-1718 bp 1.0 81.6
rps7-trnL-CAA_p4 TTAATGGATGCTGCCAGAGG CTCGCGTGTCTACCATTTCA 50.0 50.0 57.6 58.0 1681-1716 bp 1.0 81.6
rps7-trnL-CAA_p5 CAGAGGGAATGGCAATGCTA GCGTGTCTACCATTTCACCA 50.0 50.0 57.6 58.2 1664-1699 bp 1.0 80.2