Hotspot

psaC-ccsA

Genome · 105845-107780 · 1936 bp

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Pi 0.00562
MarkerSeek score 50.60
Primer available yes
Label psaC-ccsA

Feature Metadata

label_name
psaC-ccsA
feature_type
igs
region
Genome
length_bp
1936
variable_sites
37
pi
0.00562
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ccsA_p1 TTGTACCCAATGCGTACGAG GGAGCAATATGGGCCAATGA 50.0 50.0 58.0 58.0 2147-2394 bp 1.0 85.3
psaC-ccsA_p2 TTGTACCCAATGCGTACGAG CTGGAGCAATATGGGCCAAT 50.0 50.0 58.0 58.0 2149-2396 bp 1.0 85.3
psaC-ccsA_p3 TTGTACCCAATGCGTACGAG GGATCCGTTATTTGGGGGTC 50.0 55.0 58.0 58.0 1975-2222 bp 1.0 85.3
psaC-ccsA_p4 ATTCGGGGTGATCTAGAGGG GGAGCAATATGGGCCAATGA 55.0 50.0 58.0 58.0 2410-2654 bp 0.916667 81.8
psaC-ccsA_p5 ATTCGGGGTGATCTAGAGGG CTGGAGCAATATGGGCCAAT 55.0 50.0 58.0 58.0 2412-2656 bp 0.916667 81.8