Hotspot

trnR-ACG-trnN-GUU

Genome · 93832-94571 · 740 bp

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Pi 0.00324
MarkerSeek score 56.30
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
Genome
length_bp
740
variable_sites
10
pi
0.00324
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 GGGACTCTTGGGAACGAATC TCTAACCCTCCGTTGTTCCT 55.0 50.0 58.0 58.0 928-933 bp 1.0 80.9
trnR-ACG-trnN-GUU_p2 GGGACTCTTGGGAACGAATC GCTGTTAACTGACTGGTCGT 55.0 50.0 58.0 57.8 883-888 bp 1.0 79.9
trnR-ACG-trnN-GUU_p3 GGGACTCTTGGGAACGAATC TGGTCGTAGGTTCGAATCCT 55.0 50.0 58.0 58.2 870-875 bp 1.0 79.9
trnR-ACG-trnN-GUU_p4 GGGACTCTTGGGAACGAATC CTGGTCGTAGGTTCGAATCC 55.0 55.0 58.0 57.8 871-876 bp 1.0 79.5
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG TCTAACCCTCCGTTGTTCCT 55.0 50.0 57.8 58.0 885-890 bp 1.0 79.5