Hotspot

psbD-trnT-GGU

Genome · 84451-85763 · 1313 bp

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Pi 0.00322
MarkerSeek score 56.60
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1313
variable_sites
17
pi
0.00322
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CCCACAAAAACGAAACGGTC GGTTCAAGTCCGGTAAAGGG 50.0 55.0 58.2 58.2 1420-1445 bp 1.0 79.5
psbD-trnT-GGU_p2 CCCACAAAAACGAAACGGTC GGCGTAAGTCATCGGTTCAA 50.0 50.0 58.2 58.3 1433-1458 bp 1.0 78.7
psbD-trnT-GGU_p3 AACCCACAAAAACGAAACGG GGTTCAAGTCCGGTAAAGGG 45.0 55.0 57.7 58.2 1422-1447 bp 1.0 78.6
psbD-trnT-GGU_p4 CCCACAAAAACGAAACGGTC CATCGGTTCAAGTCCGGTAA 50.0 50.0 58.2 57.6 1424-1449 bp 1.0 77.9
psbD-trnT-GGU_p5 CGTAGCCAGTCATCAACAGT GGTTCAAGTCCGGTAAAGGG 50.0 55.0 57.6 58.2 1396-1421 bp 1.0 77.9