Hotspot

ycf3-psaA

Genome · 75069-75692 · 624 bp

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Pi 0.0051
MarkerSeek score 55.80
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
Genome
length_bp
624
variable_sites
12
pi
0.0051
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 CTCCGGGCAATTACATCGAA GGCCTTTAGCTAGCGTCTTT 50.0 50.0 58.1 58.0 798-808 bp 1.0 81.4
ycf3-psaA_p2 TCCGGGCAATTACATCGAAG GGCCTTTAGCTAGCGTCTTT 50.0 50.0 58.1 58.0 797-807 bp 1.0 81.4
ycf3-psaA_p3 CAGGACGCTTTGGAGAATGA GGCCTTTAGCTAGCGTCTTT 50.0 50.0 57.9 58.0 756-766 bp 1.0 81.1
ycf3-psaA_p4 GCTGCTGAGTATTGGGAACA GGCCTTTAGCTAGCGTCTTT 50.0 50.0 57.9 58.0 832-842 bp 1.0 80.8
ycf3-psaA_p5 ACAGGACGCTTTGGAGAATG GGCCTTTAGCTAGCGTCTTT 50.0 50.0 58.2 58.0 757-767 bp 1.0 80.4