Hotspot

ycf12-psbB

Genome · 53571-54251 · 681 bp

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Pi 0.0133
MarkerSeek score 64.60
Primer available yes
Label ycf12-psbB

Feature Metadata

label_name
ycf12-psbB
feature_type
igs
region
Genome
length_bp
681
variable_sites
29
pi
0.0133
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf12-psbB_p1 CCGTCAGAGTCAGAACAGTG TATGAACGCGATACCAAGGC 55.0 50.0 57.9 58.1 755-882 bp 1.0 83.6
ycf12-psbB_p2 CCGTCAGAGTCAGAACAGTG CAGAAATTAACCGGCCAGGA 55.0 50.0 57.9 57.9 791-918 bp 1.0 83.5
ycf12-psbB_p3 CCGTCAGAGTCAGAACAGTG CGGTATGAACGCGATACCAA 55.0 50.0 57.9 58.2 758-885 bp 1.0 83.4
ycf12-psbB_p4 TGAGCTGCGCAATAACTTCT CAGAAATTAACCGGCCAGGA 45.0 50.0 57.9 57.9 773-900 bp 1.0 83.2
ycf12-psbB_p5 CTAATGGGCCCGATACAACC CAGAAATTAACCGGCCAGGA 55.0 50.0 58.1 57.9 809-936 bp 1.0 83.0