Hotspot

trnV-UAC-trnH-GUG

Genome · 27688-30373 · 2686 bp

Back to Picea

Pi 0.00472
MarkerSeek score 54.00
Primer available yes
Label trnV-UAC-trnH-GUG

Feature Metadata

label_name
trnV-UAC-trnH-GUG
feature_type
igs
region
Genome
length_bp
2686
variable_sites
46
pi
0.00472
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnH-GUG_p1 TGCATGTTGGGTTCTTGGAA TTCACAATCCACTGCCTTGG 45.0 50.0 57.9 58.4 2426-2836 bp 1.0 81.7
trnV-UAC-trnH-GUG_p2 GCCCAATGCGATAAAATGCA TTCACAATCCACTGCCTTGG 45.0 50.0 57.8 58.4 2442-2852 bp 1.0 80.5
trnV-UAC-trnH-GUG_p3 ATGCATGTTGGGTTCTTGGA TTCACAATCCACTGCCTTGG 45.0 50.0 57.7 58.4 2427-2837 bp 1.0 79.9
trnV-UAC-trnH-GUG_p4 GCATGTTGGGTTCTTGGAAC TTCACAATCCACTGCCTTGG 50.0 50.0 57.6 58.4 2425-2835 bp 1.0 78.8
trnV-UAC-trnH-GUG_p5 TGCATGTTGGGTTCTTGGAA CACAATCCACTGCCTTGGT 45.0 52.6 57.9 58.0 2424-2834 bp 1.0 76.4