Hotspot

psaI-accD

Genome · 20483-21032 · 550 bp

Back to Picea

Pi 0.00641
MarkerSeek score 60.40
Primer available yes
Label psaI-accD

Feature Metadata

label_name
psaI-accD
feature_type
igs
region
Genome
length_bp
550
variable_sites
13
pi
0.00641
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-accD_p1 TTGCCGGAAGTAATAAGCCG TTGATCTGATCGTTCCACGT 50.0 45.0 58.3 57.0 675-717 bp 1.0 74.5
psaI-accD_p2 TTGCCGGAAGTAATAAGCCG CAGCTTTACAGTCTTCCTCGT 50.0 47.6 58.3 57.7 622-664 bp 1.0 72.4
psaI-accD_p3 TTGCCGGAAGTAATAAGCCG TTTGATCTGATCGTTCCACGT 50.0 42.9 58.3 57.7 676-718 bp 1.0 72.3
psaI-accD_p4 GCCGGAAGTAATAAGCCGAC TTGATCTGATCGTTCCACGT 55.0 45.0 58.8 57.0 673-715 bp 1.0 71.0
psaI-accD_p5 CCGGAAGTAATAAGCCGACT TTGATCTGATCGTTCCACGT 50.0 45.0 57.1 57.0 672-714 bp 1.0 70.2