Hotspot

ycf4-psaI

Genome · 19806-20371 · 566 bp

Back to Picea

Pi 0.00731
MarkerSeek score 59.30
Primer available yes
Label ycf4-psaI

Feature Metadata

label_name
ycf4-psaI
feature_type
igs
region
Genome
length_bp
566
variable_sites
19
pi
0.00731
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-psaI_p1 CCAGAAAGAGGATACAAGCCC CGGCTTATTACTTCCGGCAA 52.4 50.0 58.1 58.3 727 bp 1.0 70.8
ycf4-psaI_p2 CCCAGAAAGAGGATACAAGCC CGGCTTATTACTTCCGGCAA 52.4 50.0 58.1 58.3 728 bp 1.0 70.8
ycf4-psaI_p3 CCAGAAAGAGGATACAAGCCC GTCGGCTTATTACTTCCGGC 52.4 55.0 58.1 58.8 729 bp 1.0 67.2
ycf4-psaI_p4 CCCAGAAAGAGGATACAAGCC GTCGGCTTATTACTTCCGGC 52.4 55.0 58.1 58.8 730 bp 1.0 67.2
ycf4-psaI_p5 CCTAGTGAACCCAGAAAGAGG CGGCTTATTACTTCCGGCAA 52.4 50.0 57.4 58.3 737 bp 1.0 66.4