Hotspot

psbK-psbI

Genome · 7255-7742 · 488 bp

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Pi 0.0113
MarkerSeek score 42.90
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
Genome
length_bp
488
variable_sites
15
pi
0.0113
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GTTCGGTCACTATTGCGGAT 50.0 50.0 57.9 58.1 701-706 bp 1.0 81.9
psbK-psbI_p2 TCAAACTTGCCGAAGAGCTT TTCTCTTCTTCATCTGCCGC 45.0 50.0 58.0 58.0 1387-1398 bp 1.0 81.7
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTCTCTTCTTCATCTGCCGC 50.0 50.0 57.9 58.0 1148-1159 bp 1.0 81.7
psbK-psbI_p4 TCAAACTTGCCGAAGAGCTT GTTCGGTCACTATTGCGGAT 45.0 50.0 58.0 58.1 940-948 bp 1.0 81.6
psbK-psbI_p5 GAATCATCCCTTCCAACCCC TTCTCTTCTTCATCTGCCGC 55.0 50.0 57.9 58.0 1415-1426 bp 1.0 81.3