Hotspot

trnK-UUU-chlB

Genome · 4109-4901 · 793 bp

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Pi 0.00379
MarkerSeek score 59.60
Primer available yes
Label trnK-UUU-chlB

Feature Metadata

label_name
trnK-UUU-chlB
feature_type
igs
region
Genome
length_bp
793
variable_sites
9
pi
0.00379
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-chlB_p1 AGTCGCACTTAAAAGCCGAG AGGTAATGCACGCAGCTAAA 50.0 45.0 58.6 57.9 669-871 bp 1.0 79.4
trnK-UUU-chlB_p2 TAGTCGCACTTAAAAGCCGA AGGTAATGCACGCAGCTAAA 45.0 45.0 57.3 57.9 670-872 bp 1.0 78.2
trnK-UUU-chlB_p3 TCGCACTTAAAAGCCGAGTA AGGTAATGCACGCAGCTAAA 45.0 45.0 57.3 57.9 667-869 bp 1.0 78.2
trnK-UUU-chlB_p4 AGTCGCACTTAAAAGCCGAG CACGACGAAAGGGCATTTTG 50.0 50.0 58.6 58.3 702-904 bp 1.0 77.7
trnK-UUU-chlB_p5 CGCACTTAAAAGCCGAGTAC AGGTAATGCACGCAGCTAAA 50.0 45.0 57.2 57.9 666-868 bp 1.0 77.7