Hotspot

ycf1

SSC · 125099-130735 · 5637 bp

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Pi 0.00551

MarkerSeek score 55.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 31
pi: 0.00551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAACTTTCTCTATGAAATTGTCT	TGAACTCTACAATCATTGGGT	33.3	38.1	55.1	54.5	282 bp	1.0	41.1
ycf1_p2	CCAACTTTCTCTATGAAATTGTCT	TGAACTCTACAATCATTGGGTT	33.3	36.4	55.1	55.4	282 bp	1.0	41.1
ycf1_p3	CCAACTTTCTCTATGAAATTGTCT	ATGAACTCTACAATCATTGGGT	33.3	36.4	55.1	55.1	283 bp	1.0	41.1
ycf1_p4	CCAACTTTCTCTATGAAATTGTC	TGAACTCTACAATCATTGGGT	34.8	38.1	53.7	54.5	282 bp	1.0	41.1
ycf1_p5	CCAACTTTCTCTATGAAATTGTCT	TGAACTCTACAATCATTGGGTTT	33.3	34.8	55.1	56.1	282 bp	1.0	41.1