Hotspot

ndhA

SSC · 120966-123159 · 2194 bp

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Pi 0.00412

MarkerSeek score 60.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2194
variable_sites: 9
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.6	57.8	2411-2420 bp	1.0	43.5
ndhA_p2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	39.1	50.0	56.5	57.8	2414-2423 bp	1.0	42.6
ndhA_p3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2343-2352 bp	1.0	40.6
ndhA_p4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2344-2353 bp	1.0	40.6
ndhA_p5	TCATGAACTCAGTTACCATAGGAA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.1	57.8	2414-2423 bp	1.0	40.6