Hotspot

matK

LSC · 2143-3663 · 1521 bp

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Pi 0.0046

MarkerSeek score 48.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 7
pi: 0.0046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CTACTGGAGGAGCAGCAATG	CGGTACAAAACCCTGTTCGA	55.0	50.0	58.0	58.1	2777-2782 bp	1.0	78.8
matK_p2	CTACTGGAGGAGCAGCAATG	TCGGTACAAAACCCTGTTCG	55.0	50.0	58.0	58.1	2778-2783 bp	1.0	78.8
matK_p3	CTACTGGAGGAGCAGCAATG	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.0	58.2	2960-2965 bp	1.0	78.5
matK_p4	CTACTGGAGGAGCAGCAATG	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.0	58.2	2959-2964 bp	1.0	78.5
matK_p5	CTACTGGAGGAGCAGCAATG	ATACGGGAAGGACTTCTCCC	55.0	55.0	58.0	58.2	2599-2604 bp	1.0	78.2