Hotspot

trnN(GUU)-trnR(ACG)

IRa · 131136-131710 · 575 bp

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Pi 0.00348

MarkerSeek score 60.40

Primer available yes

Label trnN(GUU)-trnR(ACG)

Feature Metadata

label_name: trnN(GUU)-trnR(ACG)
feature_type: igs
region: IRa
length_bp: 575
variable_sites: 2
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-trnR(ACG)_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	715-716 bp	1.0	78.6
trnN(GUU)-trnR(ACG)_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	752-753 bp	1.0	78.0
trnN(GUU)-trnR(ACG)_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	756-757 bp	1.0	77.8
trnN(GUU)-trnR(ACG)_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	666-667 bp	1.0	77.4
trnN(GUU)-trnR(ACG)_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	793-794 bp	1.0	77.3