Hotspot

trnR(ACG)-trnN(GUU)

IRb · 110069-110643 · 575 bp

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Pi 0.00348

MarkerSeek score 60.40

Primer available yes

Label trnR(ACG)-trnN(GUU)

Feature Metadata

label_name: trnR(ACG)-trnN(GUU)
feature_type: igs
region: IRb
length_bp: 575
variable_sites: 2
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ACG)-trnN(GUU)_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	715-716 bp	1.0	78.6
trnR(ACG)-trnN(GUU)_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	752-753 bp	1.0	78.0
trnR(ACG)-trnN(GUU)_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	756-757 bp	1.0	77.8
trnR(ACG)-trnN(GUU)_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	666-667 bp	1.0	77.4
trnR(ACG)-trnN(GUU)_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	793-794 bp	1.0	77.3