Hotspot

petD-rpoA

LSC · 80116-80299 · 184 bp

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Pi 0.0166

MarkerSeek score 63.60

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 3
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TGAACATTTGCGCCTAGAAGA	45.0	42.9	57.9	57.9	330-333 bp	1.0	72.3
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TGAACATTTGCGCCTAGAAGA	45.0	42.9	57.9	57.9	331-334 bp	1.0	72.3
petD-rpoA_p3	GTCGCTCTTTGGTTGGGTAT	TGAACATTTGCGCCTAGAAGA	50.0	42.9	57.9	57.9	338-341 bp	1.0	72.1
petD-rpoA_p4	CAGTAGCGACAACCGTCTTT	TGAACATTTGCGCCTAGAAGA	50.0	42.9	58.2	57.9	373-376 bp	1.0	71.1
petD-rpoA_p5	TTGGTTGGGTATTGGTGCAA	ACAGTCCGGAAGATCTCATGA	45.0	47.6	57.9	58.3	355-358 bp	1.0	70.6