Hotspot

rpl20-rps12

LSC · 71348-72147 · 800 bp

Back to Picconia

Pi 0.00375

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 3
pi: 0.00375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	900-901 bp	1.0	75.8
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	955-956 bp	1.0	75.1
rpl20-rps12_p3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	954-955 bp	1.0	75.1
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	863-864 bp	1.0	74.3
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	917-918 bp	1.0	73.6