Hotspot

rbcL-accD

LSC · 58606-59277 · 672 bp

Back to Picconia

Pi 0.0045

MarkerSeek score 62.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 3
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACCTGCGTTCTAACTCCTTC	55.0	50.0	58.0	57.3	837-847 bp	1.0	75.0
rbcL-accD_p2	ATTTGAGTTTGCCGCAATGG	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	778-788 bp	1.0	74.7
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ACCTGCGTTCTAACTCCTTC	50.0	50.0	57.9	57.3	807-817 bp	1.0	74.4
rbcL-accD_p4	TGAGTTTGCCGCAATGGATA	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	775-785 bp	1.0	74.4
rbcL-accD_p5	AGTTTGCCGCAATGGATACT	ACCTGCGTTCTAACTCCTTC	45.0	50.0	57.9	57.3	773-783 bp	1.0	74.3