Hotspot

psbM-trnD(GUC)

LSC · 31355-31997 · 643 bp

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Pi 0.00933

MarkerSeek score 58.40

Primer available yes

Label psbM-trnD(GUC)

Feature Metadata

label_name: psbM-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 6
pi: 0.00933
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GUC)_p1	AGAATGAATAGTGCAGTCGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.9	58.5	754-756 bp	1.0	60.4
psbM-trnD(GUC)_p2	AGAATGAATAGTGCAGTCGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.9	57.0	755-757 bp	1.0	56.2
psbM-trnD(GUC)_p3	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.4	58.5	775-777 bp	1.0	55.9
psbM-trnD(GUC)_p4	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.4	58.5	767-769 bp	1.0	55.8
psbM-trnD(GUC)_p5	AGAATGAATAGTGCAGTCGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.9	57.8	753-755 bp	1.0	54.4