Hotspot

trnQ(UUG)-psbK

LSC · 8135-8469 · 335 bp

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Pi 0.012

MarkerSeek score 51.40

Primer available yes

Label trnQ(UUG)-psbK

Feature Metadata

label_name: trnQ(UUG)-psbK
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 4
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ(UUG)-psbK_p1	GACCAAAACCCGTTGCCTTA	ACTACTCGAATAAAGGGCAGA	50.0	42.9	58.7	56.2	432-433 bp	1.0	52.9
trnQ(UUG)-psbK_p2	GAATAGCGGGACCAAAACCC	ACTACTCGAATAAAGGGCAGA	55.0	42.9	58.9	56.2	441-442 bp	1.0	51.1
trnQ(UUG)-psbK_p3	GACCAAAACCCGTTGCCTTA	AACTACTCGAATAAAGGGCAGA	50.0	40.9	58.7	56.9	433-434 bp	1.0	50.8
trnQ(UUG)-psbK_p4	GACCAAAACCCGTTGCCTTA	ACTACTCGAATAAAGGGCAGAA	50.0	40.9	58.7	56.9	432-433 bp	1.0	50.8
trnQ(UUG)-psbK_p5	ATATGGCTCTGGGACGGAAG	ACTACTCGAATAAAGGGCAGA	55.0	42.9	58.9	56.2	474-475 bp	1.0	50.7