Hotspot

trnH(GUG)-psbA

LSC · 100-546 · 447 bp

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Pi 0.0112
MarkerSeek score 54.50
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
447
variable_sites
5
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 TTAGTTATGGGCGAACGACG CCGTGCTAACCTTGGTATGG 50.0 55.0 58.1 58.3 627 bp 1.0 77.2
trnH(GUG)-psbA_p2 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 572 bp 1.0 76.9
trnH(GUG)-psbA_p3 TTAGTTATGGGCGAACGACG ACCGTGCTAACCTTGGTATG 50.0 50.0 58.1 57.6 628 bp 1.0 76.8
trnH(GUG)-psbA_p4 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 573 bp 1.0 76.7
trnH(GUG)-psbA_p5 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 573 bp 1.0 76.4