Hotspot

rpl2

IRa · 154029-154853 · 825 bp

Back to Phytolacca

Pi 0.00156

MarkerSeek score 36.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 825
variable_sites: 4
pi: 0.00156
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	TCCGCCTTTGTGTTAAGCTT	50.0	45.0	58.0	58.0	1495-1508 bp	1.0	84.2
rpl2_p2	GAATGGTTAAAGCGCCCAAC	TTCCGCCTTTGTGTTAAGCT	50.0	45.0	58.0	58.0	1496-1509 bp	1.0	84.2
rpl2_p3	GAATGGTTAAAGCGCCCAAC	TGCCCGAGACCAAGTTACTA	50.0	50.0	58.0	58.1	1526-1539 bp	1.0	83.8
rpl2_p4	GCATTGGGTCGAACTCTTCT	TCCGCCTTTGTGTTAAGCTT	50.0	45.0	57.9	58.0	1161-1174 bp	1.0	83.6
rpl2_p5	GCATTGGGTCGAACTCTTCT	TTCCGCCTTTGTGTTAAGCT	50.0	45.0	57.9	58.0	1162-1175 bp	1.0	83.5