Hotspot

rpl33-rps18

LSC · 68737-68943 · 207 bp

Back to Phytolacca

Pi 0.0389

MarkerSeek score 65.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 21
pi: 0.0389
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CTAGTCGGTTGGAATTGCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.0	57.7	360-393 bp	1.0	83.9
rpl33-rps18_p2	CTAGTCGGTTGGAATTGCGA	CAAACGCCTGCGAAAAGATC	50.0	50.0	58.0	58.4	331-364 bp	1.0	83.3
rpl33-rps18_p3	CGACACAATAGGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	57.9	57.7	373-406 bp	1.0	82.8
rpl33-rps18_p4	ACACAATAGGCCTAGTCGGT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	371-404 bp	1.0	82.7
rpl33-rps18_p5	TAGGCCTAGTCGGTTGGAAT	TCAATTCGATCCCCCGATTG	50.0	50.0	57.8	57.7	365-398 bp	1.0	82.5