Hotspot

psbE-petL

LSC · 65941-66913 · 973 bp

Back to Phytolacca

Pi 0.0187

MarkerSeek score 59.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 43
pi: 0.0187
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCT	50.0	50.0	57.9	56.8	1165-1229 bp	1.0	75.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.9	56.8	1166-1230 bp	1.0	75.4
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAGACCT	50.0	47.6	57.9	58.3	1166-1230 bp	1.0	75.2
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1089-1153 bp	1.0	75.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCT	50.0	50.0	57.8	56.8	1088-1152 bp	1.0	75.0