Hotspot

petA-psbJ

LSC · 64175-65143 · 969 bp

Back to Phytolacca

Pi 0.0209

MarkerSeek score 59.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 60
pi: 0.0209
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAACTGTCCG	ATTCTGGATTGGGCTCATCC	55.0	50.0	58.6	57.3	1011-1075 bp	1.0	77.5
petA-psbJ_p2	GAGAAGGTTCAACTGTCCGA	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.2	57.3	1010-1074 bp	1.0	75.7
petA-psbJ_p3	TCGAGAAGGTTCAACTGTCC	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.2	57.3	1012-1076 bp	1.0	75.6
petA-psbJ_p4	CGAGAAGGTTCAACTGTCCG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	58.6	58.1	1060-1124 bp	1.0	73.8
petA-psbJ_p5	CGAGAAGGTTCAACTGTCCG	AGCTGGTATTCTTGTGATCGG	55.0	47.6	58.6	57.8	1061-1125 bp	1.0	73.1