Hotspot

trnM-CAU-atpE

LSC · 53500-53806 · 307 bp

Back to Phytolacca

Pi 0.0485
MarkerSeek score 65.80
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
307
variable_sites
36
pi
0.0485
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TTGACGTAGGTCTGCCTTTG CTCTCAGACGAGCTAGGACA 50.0 55.0 57.8 58.0 1013-1067 bp 0.909091 79.8
trnM-CAU-atpE_p2 TTGACGTAGGTCTGCCTTTG TACGCCTTAACGACCAATGG 50.0 50.0 57.8 58.0 1214-1268 bp 0.909091 79.2
trnM-CAU-atpE_p3 AGCCCTTGTGCTTGTGATAC TACGCCTTAACGACCAATGG 50.0 50.0 58.2 58.0 809 bp 0.272727 49.4
trnM-CAU-atpE_p4 CAGCCCTTGTGCTTGTGATA TACGCCTTAACGACCAATGG 50.0 50.0 58.2 58.0 810 bp 0.272727 49.4
trnM-CAU-atpE_p5 AGCCCTTGTGCTTGTGATAC CTCTCAGACGAGCTAGGACA 50.0 55.0 58.2 58.0 608 bp 0.272727 49.3