Hotspot

ndhC-trnV-UAC

LSC · 51388-52584 · 1197 bp

Back to Phytolacca

Pi 0.0291
MarkerSeek score 67.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1197
variable_sites
93
pi
0.0291
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1183-1394 bp 1.0 85.4
ndhC-trnV-UAC_p2 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1182-1393 bp 1.0 82.7
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1186-1397 bp 1.0 82.4
ndhC-trnV-UAC_p4 TCTGGTCCTTTGCTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1187-1398 bp 1.0 82.4
ndhC-trnV-UAC_p5 TGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1185-1396 bp 1.0 82.4