Hotspot

rps4-trnT-UGU

LSC · 46552-47061 · 510 bp

Back to Phytolacca

Pi 0.0294

MarkerSeek score 72.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 510
variable_sites: 40
pi: 0.0294
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GATCATTTCCGGCTCTAGGC	TGTAGAATTTCTGCGAGCCC	55.0	50.0	58.2	58.0	659-716 bp	1.0	86.9
rps4-trnT-UGU_p2	AAAGCCCCTAGACGACGTAT	TGTAGAATTTCTGCGAGCCC	50.0	50.0	58.2	58.0	618-675 bp	1.0	86.9
rps4-trnT-UGU_p3	AAGCCCCTAGACGACGTATT	TGTAGAATTTCTGCGAGCCC	50.0	50.0	58.2	58.0	617-674 bp	1.0	86.9
rps4-trnT-UGU_p4	AGCCCCTAGACGACGTATTT	TGTAGAATTTCTGCGAGCCC	50.0	50.0	58.2	58.0	616-673 bp	1.0	86.9
rps4-trnT-UGU_p5	AACGCGGTTGGTTTCTAAGA	TGTAGAATTTCTGCGAGCCC	45.0	50.0	57.8	58.0	677-734 bp	1.0	86.4