Hotspot

psaA-ycf3

LSC · 42284-42794 · 511 bp

Back to Phytolacca

Pi 0.0281

MarkerSeek score 57.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 32
pi: 0.0281
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGTTTCAGGGCCCTTAGCTA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	735-786 bp	1.0	86.9
psaA-ycf3_p2	TAGTTTCAGGGCCCTTAGCT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	736-787 bp	1.0	86.9
psaA-ycf3_p3	GTAGTTTCAGGGCCCTTAGC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	737-788 bp	1.0	86.8
psaA-ycf3_p4	GCATCAGCATGTAGGTTCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	767-818 bp	1.0	86.7
psaA-ycf3_p5	CCGCCTACATCACCATTCAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	998-1049 bp	1.0	85.6