Hotspot

trnD-GUC-trnY-GUA

LSC · 31008-31490 · 483 bp

Back to Phytolacca

Pi 0.0198
MarkerSeek score 61.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
483
variable_sites
27
pi
0.0198
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 522-553 bp 1.0 82.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 521-552 bp 1.0 82.3
trnD-GUC-trnY-GUA_p3 ATTGGGTATTGGATCCGTCG TGGCAATATGTCTACGCTGG 50.0 50.0 57.5 57.8 584-615 bp 1.0 81.5
trnD-GUC-trnY-GUA_p4 ATTGGGTATTGGATCCGTCG GGCAATATGTCTACGCTGGT 50.0 50.0 57.5 57.8 583-614 bp 1.0 81.4
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 543-574 bp 1.0 81.2