Hotspot

psbM-trnD-GUC

LSC · 29941-30933 · 993 bp

Back to Phytolacca

Pi 0.0114

MarkerSeek score 62.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 32
pi: 0.0114
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTTTGGCTGACCGTTTT	GTTCCCGGGGATTGTAGTTC	40.0	55.0	56.4	58.0	1148-1177 bp	1.0	71.8
psbM-trnD-GUC_p2	AATCATTTTGGCTGACCGTT	GTTCCCGGGGATTGTAGTTC	40.0	55.0	56.2	58.0	1150-1179 bp	1.0	69.9
psbM-trnD-GUC_p3	ATCATTTTGGCTGACCGTTT	GTTCCCGGGGATTGTAGTTC	40.0	55.0	56.2	58.0	1149-1178 bp	1.0	69.9
psbM-trnD-GUC_p4	TCATTTTGGCTGACCGTTTTT	GTTCCCGGGGATTGTAGTTC	38.1	55.0	57.2	58.0	1148-1177 bp	1.0	69.8
psbM-trnD-GUC_p5	TCATTTTGGCTGACCGTTTT	AGTTCCCGGGGATTGTAGTT	40.0	50.0	56.4	58.3	1149-1178 bp	1.0	69.4