Hotspot

psbI-trnS-GCU

LSC · 8078-8281 · 204 bp

Back to Phytolacca

Pi 0.026

MarkerSeek score 56.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 16
pi: 0.026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTAAG	GCTTTGGGGCGAAGAAGTAT	50.0	50.0	57.9	58.2	1335-1405 bp	1.0	86.1
psbI-trnS-GCU_p2	CACAATCCGGATCACCTTGT	GCTTTGGGGCGAAGAAGTAT	50.0	50.0	57.9	58.2	1181-1251 bp	1.0	85.7
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTAAG	AGGCCGCGGGAATAATAAAA	50.0	45.0	57.9	57.6	1364-1434 bp	1.0	85.2
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	ATCCGTTGTACGACTCGTTC	50.0	50.0	57.9	57.7	821-865 bp	0.636364	68.6
psbI-trnS-GCU_p5	CACAATCCGGATCACCTTGT	ATCCGTTGTACGACTCGTTC	50.0	50.0	57.9	57.7	667-711 bp	0.636364	68.0