Hotspot

ndhG-ndhI

SSC · 121180-121589 · 410 bp

Back to Physospermopsis

Pi 0.032

MarkerSeek score 65.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 410
variable_sites: 36
pi: 0.032
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CATTTATGGCCCCCACGTAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.9	58.1	1062-1125 bp	1.0	86.4
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1364-1427 bp	1.0	85.7
ndhG-ndhI_p3	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1363-1426 bp	1.0	85.7
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	906-969 bp	0.75	76.0
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	905-968 bp	0.75	76.0