Hotspot

rpl32-trnL-UAG

SSC · 115616-116566 · 951 bp

Back to Physospermopsis

Pi 0.028
MarkerSeek score 71.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
951
variable_sites
75
pi
0.028
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTTGGAAAGGGGAGGGGTA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1052-1111 bp 1.0 89.1
rpl32-trnL-UAG_p2 TATTTGGAAAGGGGAGGGGT AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1053-1112 bp 1.0 89.1
rpl32-trnL-UAG_p3 TTGGAAAGGGGAGGGGTATT AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1050-1109 bp 1.0 89.1
rpl32-trnL-UAG_p4 TTTGGAAAGGGGAGGGGTAT AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1051-1110 bp 1.0 89.1
rpl32-trnL-UAG_p5 TTGGAAAGGGGAGGGGTATT CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1051-1110 bp 1.0 89.1