Hotspot

rps12-clpP

LSC · 71440-71597 · 158 bp

Back to Physospermopsis

Pi 0.0266

MarkerSeek score 68.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 10
pi: 0.0266
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GTACATGTTCCTCGACGCTG	TGATCTTGTAGCGGTGGTTG	55.0	50.0	58.7	57.9	271-289 bp	1.0	80.3
rps12-clpP_p2	GTACATGTTCCTCGACGCTG	TGTTGATCTTGTAGCGGTGG	55.0	50.0	58.7	57.9	274-292 bp	1.0	80.2
rps12-clpP_p3	GTACATGTTCCTCGACGCTG	GTTGATCTTGTAGCGGTGGT	55.0	50.0	58.7	57.9	273-291 bp	1.0	80.2
rps12-clpP_p4	GTACATGTTCCTCGACGCTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.7	57.9	307-325 bp	1.0	79.8
rps12-clpP_p5	AGTACATGTTCCTCGACGCT	TGATCTTGTAGCGGTGGTTG	50.0	50.0	58.8	57.9	272-290 bp	1.0	79.5