Hotspot

ndhC-trnV-UAC

LSC · 51936-52660 · 725 bp

Back to Physospermopsis

Pi 0.0432

MarkerSeek score 79.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 75
pi: 0.0432
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	682-812 bp	1.0	51.9
ndhC-trnV-UAC_p2	TGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	56.3	58.1	731-861 bp	1.0	51.4
ndhC-trnV-UAC_p3	TGCCAAAATAGGAATAAGACTTG	CCGAGAAAGTCTACGGTTCG	34.8	55.0	54.9	58.1	731-861 bp	1.0	51.4
ndhC-trnV-UAC_p4	ATGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	32.0	55.0	56.8	58.1	732-862 bp	1.0	51.4
ndhC-trnV-UAC_p5	TGCCAAAATAGGAATAAGACTTGAT	CCGAGAAAGTCTACGGTTCG	32.0	55.0	56.8	58.1	731-861 bp	1.0	51.4