Hotspot

trnF-GAA-ndhJ

LSC · 49842-50259 · 418 bp

Back to Physospermopsis

Pi 0.0347

MarkerSeek score 64.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 24
pi: 0.0347
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	446-474 bp	1.0	48.2
trnF-GAA-ndhJ_p2	CTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	57.9	53.8	470-498 bp	1.0	48.0
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	482-510 bp	1.0	47.9
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATGAAATACAAGATGCTCATTGA	55.0	30.4	58.0	54.4	483-511 bp	1.0	47.9
trnF-GAA-ndhJ_p5	GAATGGTCGGGATAGCTCAG	TGAAATACAAGATGCTCATTGA	55.0	31.8	57.6	53.8	490-518 bp	1.0	47.8