Pi
0.0278
MarkerSeek score
69.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 815-859 bp | 1.0 | 88.3 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 847-891 bp | 1.0 | 87.2 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ATTAAGTCCGCAGCGTCTAC | 55.0 | 50.0 | 57.9 | 57.8 | 833-877 bp | 1.0 | 86.5 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ATTAAGTCCGCAGCGTCTAC | 50.0 | 50.0 | 57.8 | 57.8 | 865-909 bp | 1.0 | 85.5 |
| trnT-UGU-trnL-UAA_p5 | ATGCGATGCTCTAACCTCTG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.5 | 58.0 | 817-861 bp | 1.0 | 85.1 |