Hotspot

trnS-UGA-psbZ

LSC · 36745-37119 · 375 bp

Back to Physospermopsis

Pi 0.0342

MarkerSeek score 70.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 28
pi: 0.0342
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ACAGCCAATTGGAAAGCAAG	55.0	45.0	58.0	57.2	409-443 bp	1.0	82.5
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	ACAGCCAATTGGAAAGCAAG	55.0	45.0	58.1	57.2	416-450 bp	1.0	82.2
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	ACAGCCAATTGGAAAGCAAG	55.0	45.0	58.1	57.2	417-451 bp	1.0	82.2
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	ACAGCCAATTGGAAAGCAAG	55.0	45.0	58.1	57.2	424-458 bp	1.0	82.0
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.0	57.0	416-450 bp	1.0	81.1