Hotspot

trnT-GGU-psbD

LSC · 32566-33987 · 1422 bp

Back to Physospermopsis

Pi 0.0235

MarkerSeek score 68.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1422
variable_sites: 92
pi: 0.0235
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TCAAATCCGATAAGGGGCT	AGACCGGACCATCCTACAAA	47.4	50.0	55.7	58.1	1504-1562 bp	1.0	62.3
trnT-GGU-psbD_p2	TCAAATCCGATAAGGGGCT	GAGCAATAGACCGGACCATC	47.4	55.0	55.7	57.8	1511-1569 bp	1.0	61.5
trnT-GGU-psbD_p3	TCAAATCCGATAAGGGGCT	AGAGCAATAGACCGGACCAT	47.4	50.0	55.7	58.2	1512-1570 bp	1.0	61.1
trnT-GGU-psbD_p4	TCAAATCCGATAAGGGGCT	ACAAAAACGAAACGGTCCCT	47.4	45.0	55.7	58.2	1489-1547 bp	1.0	60.8
trnT-GGU-psbD_p5	TCAAATCCGATAAGGGGCT	CAAAAACGAAACGGTCCCTC	47.4	50.0	55.7	57.6	1488-1546 bp	1.0	59.6