Hotspot

petN-psbM

LSC · 29394-30489 · 1096 bp

Back to Physospermopsis

Pi 0.0203

MarkerSeek score 64.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 58
pi: 0.0203
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CCCTTTCACTCGTCGTATGG	ACAGAAACTCAAAATAAAAAGGA	55.0	26.1	58.1	52.7	1153-1183 bp	0.5	25.0
petN-psbM_p2	CCTTTCACTCGTCGTATGGG	ACAGAAACTCAAAATAAAAAGGA	55.0	26.1	58.1	52.7	1152-1182 bp	0.5	25.0
petN-psbM_p3	CGTCGTATGGGGAAGAAGTG	ACAGAAACTCAAAATAAAAAGGA	55.0	26.1	58.1	52.7	1143-1173 bp	0.5	25.0
petN-psbM_p4	CTTTCACTCGTCGTATGGGG	ACAGAAACTCAAAATAAAAAGGA	55.0	26.1	58.1	52.7	1151-1181 bp	0.5	25.0
petN-psbM_p5	TCGTCGTATGGGGAAGAAGT	ACAGAAACTCAAAATAAAAAGGA	50.0	26.1	58.2	52.7	1144-1174 bp	0.5	25.0