Hotspot

rps2-rpoC2

LSC · 16774-16991 · 218 bp

Back to Physospermopsis

Pi 0.0334

MarkerSeek score 68.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 20
pi: 0.0334
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCGGCCTTCATC	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	887-898 bp	1.0	84.2
rps2-rpoC2_p2	CCGCATCTCTTTTCGGAAGA	TAGCAAAAGCAGCTCTACGG	50.0	50.0	58.0	58.0	903-914 bp	1.0	84.1
rps2-rpoC2_p3	AACATAATGACACCGAGCCC	TAGCAAAAGCAGCTCTACGG	50.0	50.0	58.0	58.0	776-787 bp	1.0	84.1
rps2-rpoC2_p4	CCGCATCTCTTTTCGGAAGA	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	1244-1255 bp	1.0	83.6
rps2-rpoC2_p5	AACATAATGACACCGAGCCC	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	1117-1128 bp	1.0	83.5